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Enzyme Activity Ia Essays

Enzyme Activity Ia Essays Enzyme Activity Ia Essay Enzyme Activity Ia Essay Year 11 IB Biology IA Report 2011 5/31/2011 Enzyme Activity Experiment Introduction3 AIM3 Materials3 Method4 Result5 Table 1:5 Table 2:5 Graph 16 Discussion6 Conclusion8 Bibliography9 Appendix 1 (Test tube 3- 45 degrees)9 Appendix 2 (Test tube 1- 5 degrees)9 Appendix 3 (Test tube 2- 20 degrees)9 Introduction Phenolphthalein is an indicator that is pink in alkaline solutions of about pH10. When the pH drops below pH 8. 3 phenolphthalein will go colourless. Here, an alkaline solution of milk, lipase and phenolphthalein will change from pink to colourless as the fat in milk is broken down to form fatty acids (and glycerol) thus reducing the pH to below 8. 3. The time taken for this reaction to occur is affected by temperature. AIM To investigate the effects of increasing temperature on the activity of enzyme Lipase. Materials * Milk, full-fat or semi-skimmed, 5 cm3 per student per temperature assessed * Phenolphthalein in a dropper bottle (see note 1) * 5% lipase solution, 1 cm3 per student per temperature assessed * Sodium carbonate solution, 0. 05 mol dm-3, 7 cm3 per student per temperature assessed * Electric hot water baths set to a range of temperatures, each containing a thermometer, a test-tube rack and a beaker of lipase solution. * Ice * Marker pen * Test tube rack * Measuring cylinder (or syringe), 10 cm3, 2 * Beaker, 100 cm3, 2 (for milk and sodium carbonate solution) * Beaker, 250 cm3, 2 (to act as water baths for temperatures below room temperature) * Thermometer * Test tube * Glass rod * Syringe, 2 cm3 * Stop clock/stopwatch Method 1. Label a test tube with the temperature to be investigated. 2. Add 5 drops of phenolphthalein to the test tube. 3. Measure out 5 cm3 of milk using a measuring cylinder (or syringe) and add this to the test tube. 4. Measure out 7 cm3 of sodium carbonate solution using another measuring cylinder (or syringe) and add this to the test tube. The solution should be pink. 5. Place a thermometer in the test tube. Take care as the equipment could topple over. 6. Place the test tube in a water bath and leave until the contents reach the same temperature as the water bath. . Remove the thermometer from the test tube and replace it with a glass rod. 8. Use the 2 cm3 syringe to measure out 1 cm3 of lipase from the beaker in the water bath for the temperature you are investigating. 9. Add the lipase to the test tube and start the stopclock/ stopwatch. 10. Stir the contents of the test tube until the solution loses its pink colour. 11. Stop the clock/ watch and note the time in a suitable table of r esults. Result Table 1: | Test tube 1 5 degrees| Test tube 220 degrees| Test tube 345 degrees| Test tube 465 degrees| Time (min: sec: usec)| 31:04:86 ()| 16:26:17| 2:09:03| 32:20:74 ()| Notes| The test tube was breakdown before the liquid (milk) turns white| Complete| Complete| The liquid didn’t turn white, and colour of the liquid (Milk) was light pink| Table 2: Time (Min)| Test Tube 15 degrees| Test tube 220 degrees| Test tube 345 degrees| Test tube 465 degrees| 00:00:00| | | Light pink| | 02:00:00| | Pink | Completed| | 04:00:00| | | | Turn light pink| 06:00:00| | Light pink| | | 08:00:00| Turned light pink| | | | 10:00:00| | | | lighter| 2:00:00| | White with Pink| | | 14:00:00| Lighter| | | White with pink| 16:00:00| | Completed| | | 18:00:00| | | | | 20:00:00| | | | | 22:00:00| | | | | 24:00:00| | | | | 26:00:00| Really Light pink| | | | 28:00:00| White with pink| | | | 30:00:00| | | | | Graph 1 Discussion As the introduction of this report shown that the phenolphthalein is an indicator that is pink solution of about pH10, and when the pH drops below pH8. 3 phenol phthalein goes colourless. Through this report, the aim is to investigate the effect of increasing temperature on the activity of the enzyme lipase. The data of the experiment results showed that the sample [Test tube 1] in five degrees took around 31 minutes and 04 seconds to turns into light pink, but the testing wasn’t complete, as the test tube was break down before it completed, but record from another group’s result, it took around 50 minutes for the sample to completely turn white. (Result from Sumin’s group). The sample [Test tube 2] in 20 degrees took around 16 minutes and 26 seconds to complete the reaction and completely turned white. The sample [Test tube 3] took around 2 minutes and 09 seconds to complete the reaction and this sample took the shortest time to complete the reaction. The sample [Test tube 4] took around 32 minutes and 21 seconds to turn into very light pink (White with pink), but it didn’t complete the reaction as the time was run out. As the result, it shows that the temperature between 20 to 45 degrees is the best environment for the fat in milk to broken down to fatty acids and glycerol thus reducing the pH to below 8. and the taken time will increase if the temperature is over 45 degrees and the lipase might not break down the fat in milk or not work at all. The temperature affected the action of the fat breaks down to fatty acids and glycerol (lipase) in this way because when the temperature increase and then the time taken for the reaction will also increase by increase the collision rate between the substrate molecules and enzymes. The fastest time for the reaction is at the suitable and advantageous temperature for the enzymes which is between 20 to 45 degrees (Â ± 5 degrees). The time taken for the reaction will decrease from 5 degrees to 45 degrees, but the time will increase since the temperature is reach and over 45 degrees, as the protein structure of the enzyme will denatured by the high temperature and the enzyme is de-activated and the molecule is lose its shape. (University of Maryland Medical Center, 2009) Recording from the experiment result, the best temperature for the fat in milk breaks down to fatty acids and glycerol is between 20 to 45 degrees and it might not work really well when the temperature is over 45 degrees. On another hand, there are some weaknesses need to be improve in the experiment. Firstly, the water temperature for the Test tubes to stay in was under/ over the temperature that was suggest in the experiment as the temperature of the water was hard to control as the exactly temperature that the instruction suggested, so the group was recommend that a member from the group needs to add extra hot water/ extra ice when the temperature of the water is under/ over the temperature recommended. Secondly, the group set up and put all the test samples in the test tube at the beginning of the experiment and suddenly left the samples in the room temperature for almost 10 minutes before the group start the experiment; therefore, the results from the experiment might be wrong as the solution in the test tube might affects the lipase during the break time. As the result, the group suggest to mix up the solution and the test samples before the experiment starts, and the break time gap is not allowed. Thirdly, the syringe wasn’t used to measure out the lipase, so the group chose to restart the experiment as the results may have changed. However, after restarting the experiment as a whole, the experiment was a lot more successful and there was more care. Finally, these improvements will be use as a reference in the next class experiment. Conclusion In conclusion, the results from the experiment shown that the best temperature for the fat in milk to break down to fatty acids and glycerol is 45 degrees and the reaction will not work really well since the temperature are over 45 degrees. Also, recording from the graph, the test tube 3 (45 degrees) spent less time to complete the process and the test tube 4 (60 degrees) spent more time to turn the colour of the liquid into light pink and the process/ reaction didn’t complete in the end. As well, the information shown at the website of the University of Maryland Medical Centre displayed that the protein structure of the enzyme will denatured by the high temperature and the enzyme is deactivated and the molecule is lose its shape when the temperature is over 45 degrees. University of Maryland Medical Center, 2009). As the result, when the temperature increase from zero degrees to 45 degrees, the time taken for the reaction will decrease, but the reaction starts slow down since the temperature is reach and over 45 degrees. Bibliography University of Maryland Medical Center. (2009). Retrieved from Lipase: umm. edu/altmed/articles/lipase-000311. htm Appendix Appendix 1 (Test tube 3- 45 degrees) Appendix 2 (Test tub e 1- 5 degrees) Appendix 3 (Test tube 2- 20 degrees)

Role of the State in Internet Privacy Protection Essay

Role of the State in Internet Privacy Protection - Essay Example To specify, information privacy is believed to exist if one is able to monitor the usage of personal information, its circulation, and release (Culnan 341). With the rapid growth of the web space and technology advancement, concerns of users’ about personal privacy threats are also growing. Research into the issue found that internet users today are exposed to embarrassment, stalking, cyber-bullying, blackmailing, and identity hacking due to users’ displaying lots of personal data. This has led many people believe that internet privacy does not exist at all and is impossible to control. Others believe that the issue of internet privacy is not that important and should be regulated by the market. In my view, internet privacy exists once it is protected by the law. Many people believe that maintaining privacy on the internet and particularly while visiting social networking web sites is their basic human right. So they suppose that the basic thing they need to do to prote ct their privacy is to simply adjust the privacy settings, Steven Rambam and other interested authors think that privacy does not at all exist on the internet. Rambam, a private investigator and director of Pallorium Investigative Agency, expressed these ideas in a series of talks at U.S. conferences. His basic idea is â€Å"Privacy is Dead – Get Over It†. ... in a few hours about an individual unfamiliar to him (Rambam, â€Å"Privacy is Dead – Get Over It†) At the Last HOPE Conference, Rambam discussed the process of searching for necessary information on the internet via intrusion into privacy, which as the investigator notes â€Å"is out of the bottle† (Mills, â€Å"The Internet – a Private Eye’s Best Friend†). Rambam argues that each new search through Google, every blog post, and each new photo posted online mean further losing the battle led for privacy protection. This can be explained by the fact that â€Å"anything you put on the internet will be grabbed, indexed, cataloged, and out of your control before you know it† (Mills, â€Å"The Internet – a Private Eye’s Best Friend†). Rambam specifies that every kind of information online is digitized, with older information scanned and placed online. Afterwards, this mixture gets aggregated into special databases which are later sold to government agencies, marketers, and practically anyone capable of purchasing it. Twitter, cell phones, taking photos by iPods, etc are effective tools for identifying individual’s location, preferences in buying and similar information is collected by special marketing databases that are usually bought by the government. Due to the use of the information supplied by consumer databases, individuals are tracked down by the police, different collection agencies, and the U.S. Marshall’s Service (Mills, â€Å"The Internet – a Private Eye’s Best Friend†). The question arises then: what to do about this? It seems Rambam’s advice is to just â€Å"get over† the reality that privacy is dead on the internet. Next, many researchers acknowledge that privacy is under threat on the internet and suggest how to protect it. Specifically, the

Compare and contrast the yellow wallpaper and the ones who walk away Essay

Compare and contrast the yellow wallpaper and the ones who walk away from omelas - Essay Example Nonetheless, this peculiar work has received recognition and won the Hugo, as the most prestigious science fiction award; thus, having a frequent anthology both in main stream anthropology and science fiction. On the other hand, the story â€Å"the yellow wallpaper† is actually a renowned short story. It is interesting to note that it was just recalled from the oblivion (Costello and Tucker 475). Charlotte Perkins Gilman wrote this short story. The facts behind its original creation and its modern or current rediscovery are worth recounting. Additionally, the â€Å"the yellow wallpaper† is a true story based on the author’s personal life experiences. Notably, the story is deliberately based on her debilitating and depression effects of her medical conditions and subsequent treatment (Costello and Tucker 474). The story â€Å"the yellow wallpaper† claims to â€Å"save people from being driven crazy.† In the same way, the story â€Å"The Ones Who Walked Away From Omelas† claims civilizations for the society it is addressing. However, an auditor of the Atlantic Monthly turned the story down claiming that it contained a start and unsettling content. I n this case, both stories share a common negative publicity, in that, the classification of â€Å"The Ones Who Walked Away From Omelas† proved to be a challenge, and consequently, in the case of â€Å"the yellow wallpaper†, there is a claim that the story is a start that has unsettling content. Moreover, the story received a sentiment that it was â€Å"too terribly good to be printed.† However, following series of events on the same story, especially its discussion on the title â€Å"the Madwoman in the Attic,† led to its wide recognition and subsequent discussions within the American literature (Costello and Tucker 478). The story â€Å"The Yellow Wallpaper† has different readings and interpretations; however, it obviously invites critics who may demand a feminist approach in discussing the story.

How conservative was Hogarth's view of London Essay

How conservative was Hogarth's view of London - Essay Example Hogarth’s important works: Hogarth’s paintings portray a view of London streets that is bustling and chaotic, such that a viewer looking at the paintings gets a feeling almost of claustrophobia because they appear to be so packed and close. The angle and view which the artist presents make the view feel as if s/he is walking through crowded streets in London, as opposed to neat and well ordered ones. This feeling of being crowded and congested may be noted especially in his work titled â€Å"Southwark Fair (the humours of a fair) which is dated January 1733. This particular painting is centred around the church of St. George in Southwark. This particular painting may be a follow on of the series of eight paintings which comprised the series known as the â€Å"rake’s progress†. These were first presented by Hogarth as canvases and depicted the life of Tom Rakewell , the son of a rich merchant, who comes to London and spends away all his money on prostituti on and gambling, so that he is finally sent off to prison and on to bedlam. The last painting in the series shows an insane and violent Tom consigned to a mental hospital and the painting titled â€Å"Southwark fair† might represent Tom’s descent into bedlam.. ... As Brennan (2009) has pointed out, one out of every five women in London during that time period were prostitutes. There was a high demand for virgins and the noblemen paid a high price for the privilege of deflowering virgins. Hogarth’s portrayal of these prostitutes in his works, as also further detailed below, points out that monetary considerations took precedence over morality and virtue. Young women could earn much higher sums of money by selling out their bodies to high paying men, as opposed to plying an honest trade and working as maids or in similar professions. Hence, greed was the sole motive for the eschewing of virtue and Hogarth’s portrayal of these prostitutes is such that a fall is inevitably associated with the lives of these harlots, after years of fattening of their wallets through their earnings. Hogarth’s works of art question the existing moral standards in the London of his day. In an exhaustive discussion of black slaves and the moral and social connotations that were associated with the training of the former slaves into docile houseboys, Molineaux has noted that owning a black houseboy had become equivalent to owning a pet or a dog, so that the sight of white ladies with their black slaves had become fairly common in London. In his series of works titled â€Å" A harlot’s progress† that consist of six different plates, Hogarth details the story of a young woman who arrives in London and descends into prostitution. The second print shows the harlot in luxurious surroundings, ensconced in a parlor owned by a rich Jewish merchant, where the harlot is also attended by a monkey and a black slave, thereby signifying in a satirical manner, the so called â€Å"high standing† that this woman has attained by virtue of being the rich Jewish

A Separate Peace John Knowles English Literature Essay

A Separate Peace John Knowles English Literature Essay B. I dont think this place the story could take place at any other time because the timing of this was perfect because the timing tied in with the story and so if you had it during like the Vietnam War it couldnt have tied in with the story. C. Yes, the setting does change throughout the story the story goes from the school Devon, to the beach, to when Gene left the school to go back home, to when he went to Finnys house, and then he went back to Devon. D. The reason for the changes are that Gene and Finny decided to go to the beach even though they werent allowed to and the reason Gene left school was because it was a break and Gene went to Finnys house because he wanted to explain why he jounced the tree limb, and lastly he went back to Devon to finish his school year and graduate. 6. The basic plot of the story is that a boy named Gene who is smart and there is an athletic boy named Finny. Gene and Finny become best friends. Eventually, Gene gets jealous of Finnys athletic abilities and so he thinks theres a rivalry between them. Then he finds out that there isnt one between them and when he finds that out he is about to go jump off the tree for his secret club and so he shakes the branch that him and Finny are on and so Finny falls of the tree and break his bone. Then, Gene finds out that Finny cant play sports anymore and then he feels bad. Then the summer session is over and so Gene goes home and when he is coming back to Devon he goes to Finnys house to say sorry to him but Finny denies that Gene did it on purpose. After that, Gene comes back to Devon. Then Finny decides that Gene is going to take his place in athletics and so Finny starts training Gene for the Olympics. After that happens Brinker holds a trial because he thinks Gene did push Finny off the big tree. While the trial is going on Finny realizes that Gene really did push him off the tree on purpose and so Finny is walks out of the room and as he is walking he falls on the staircase and breaks his leg again. Then Finny dies while being operated on because some of his bone marrow goes into his blood stream. Then Gene thinks about all the things Finny had taught him and then the book ends. 7. A. The opening scene of the book is Gene that is older having a flashback and thinking about how his life was during his school years. Also, the opening scene includes Finny and Gene not coming to dinner and then being caught for skipping dinner but then Finny talks his way out of trouble. B. What is most important about the opening scene is the part where Gene says that he was changed at Devon because that is a fundamental part in the story because it shows that Gene likes this school a lot and that he has been through a lot at this school. C. The opening scene does set the stage for the rest of the book because in the opening scene it tells you about how Gene was at Devon and how he went through many difficult times at that school and so you know that the school is going to be an important part in the story. 8. A. The closing scene of the book is when Finny dies from the bone marrow getting into the blood stream. The rest of the closing scene is when Gene thinks about all the things that Finny had taught him and how he wouldnt be the same if he hadnt met Finny. B. What is most important about the closing scene is the part where Gene thinks about all the things Finny had taught him. The reason for this being the most important is because since he was remembering things about Finny it shows that he really does like Finny and that he doesnt hate Finny it shows that he does feel guilty about causing Finnys death. C. I think the closing scene does fit with the theme of the work because the theme of this book is dealing with having feelings that you never thought that you had and this fits with the closing scene because in the closing scene Finny dies because of his broken bones bone marrow and the reason Finny had broken his bone again was because he realized that Gene did negative feelings towards him and that Gene had pushed him off the tree on purpose and when he was deep in thought about that he didnt notice he was on by the staircase and he fell down it because he had been too busy thinking about Genes true feelings. 9. A. The main characters in the story are Finny and Gene. What distinguishes Finny from everyone else is that he is very athletic and what distinguishes Gene from everyone else is that he is much more intelligent than most people. B. The characters are believable because there are many people in the world who are much more intelligent than others and there are also many people who are much more athletic than others so they are believable characters. C. An important minor character is Brinker. The purpose Brinker serves is to be like a literary foil of Finny and he is the one who decides to have a trial about the cause of Finnys accident. Another important minor character is Leper who is a peaceful guy and because of him going to war early and coming back insane Finny is forced to believe in the war and that is his purpose in the story. 10. A. The basic conflict in this book is that Gene becomes jealous of Finny and then makes Finny fall off a tree and break his bone because he is jealous. The reason Gene is jealous of Finny is because of Finnys amazing athleticism and his great personality. B. The major conflict in this story is an internal conflict. C. There are many other conflicts in this story. One minor conflict in this story is World War II. World War II goes on all throughout the book and it is constantly used by the author. Another minor conflict in this story is that Gene doesnt want to enlist in the war and so he is the only one in his grade who hadnt enlisted. 11. The theme of this book is betrayal. 12. The major symbols of this book are the big tree that Gene and Finny jumped off of B. The big tree stands for revenge to me because the big tree is where Gene made Finny break his bone because Gene was jealous of Finny. 13. The title means that there are different types of peace you can have. For example, you can have peace like when you end a war and there is peace or when you are calm and nonviolent that can be peaceful too. I think this title means that there is going to be a separate type of peace apart from the peace after the war that is going on. B. The significant clues that the title gives to the meaning of the story are that from the title you know that the story isnt going to be about the peace after the war it is going to be about peace between something on a smaller scale like between two people. C. Yes the title is related to one of the aspects of the book. The aspect of the book that the title is related to is the setting because the setting is Devon during WWII and when there is a world war going on in the book you would think that the peace was going to be about the war but the title says separate so then you understand that it means a peace that is not from the war. 14. A. The tone of the book is a sad and guilty tone. B. The tone of the book changes from being happy in the beginning to sad and guilty after the climax to resentment at the end of the book. C. The passages that you can figure out the tone of the book are the passage after Finny falls off the tree and the passage at the end where it talks about how Gene remembering all the things he had learned from Finny. 15. The time of this novel is mostly flashbacks because from page fourteen to about the end of the book because in the beginning he goes to the school and then he remembers all the things that he had done in the school and everything he remembers is one big flashback and that flashback is the book. 16. The main characteristics of the authors style are varying sentence length, using similes, and only having a first person point of view. 17. I think the author wrote this book because he wanted to entertain readers and because he wanted to teach people about how people really are even though. I think he is trying to say that even if people seem nice you can never really tell if they are or not. B. The ultimate purpose the author hoped to accomplish by writing this book is to show the reader that during the war was going on there were many other struggles people were going through.

Terrorism and the Just War Tradition Essay -- September 11 Terrorism E

Terrorism and the Just War Tradition       It ´s important, at the outset, to understand what the just-war tradition is, and isn ´t. The just-war tradition is not an algebra that provides custom-made, clear-cut answers under all circumstances. Rather, it is a kind of ethical calculus, in which moral reasoning and rigorous empirical analysis are meant to work together, in order to provide guidance to public authorities on whom the responsibilities of decision-making fall. This essay will study the tradition and apply it to the Sept. 11 aftermath.    From its beginnings in St. Augustine, just-war thinking has been based on the presumption -- better, the classic moral judgment -- that rightly-constituted public authorities have the moral duty to pursue justice -- even at risk to themselves and those for whom they are responsible. That is why, for example, St. Thomas Aquinas discussed just war under the broader subject of the meaning of "charity," and why the eminent Protestant theologian Paul Ramsey argued that the just-war tradition is an attempt to think through the public meaning of the commandment of love-of-neighbor. In today ´s international context, "justice" includes the defense of freedom (especially religious freedom), and the defense of a minimum of order in international affairs. For these are the crucial components of the peace that is possible in a fallen world.    This presumption -- that the pursuit of justice is a moral obligation of statecraft -- shapes the first set of moral criteria in the just-war tradition, which scholars call the "ius ad bellum" or "war-decision law:" Is the cause a just one? Will the war be conducted by a responsible public authority? Is there a "right intention" (which, among ... ...tc.; no one suggests that guerrilla warfare is anything other than warfare. It is true that the just-war tradition is accustomed to thinking of states as the only "unit-of-count" in world politics. The new situation demands a development of the just-war tradition. As a method of moral reasoning about politics, the just-war tradition emerged long before the state system; the tradition developed to deal with the realities of a world in which states were the primary actors, and now it must develop to help us think through our moral obligations in a world in which non-state actors, like terrorist organizations and networks (often allied with states), are crucial, and intentionally lethal, actors.    SOURCES CITED:    CNN.com http://www.cnn.com/    In Response to Terrorism. http://www.powertochange.com/peace/articles/terror_response.html